ea0065p365 | Reproductive Endocrinology and Biology | SFEBES2019
Faqehi Abdullah
, Denham Scott
, Naredo-Gonzalezb Gregorio
, Cobice Diego
, Sabil Ghazali
, Upreti Rita
, Gibb Fraser
, Homer Natalie
, Andrew Ruth
Liquid Chromatography tandem mass spectrometry (LCMS/MS) is gold-standard for androgen analysis in biological fluids, superseding immunoassays in specificity, particularly at low concentrations. While LCMS/MS is well established for analysis of testosterone (T) and androstenedione (A4), 5α-dihydrotestosterone (DHT) presents greater analytical challenges. DHT circulates at low nanomolar concentrations in men and lower in women, ionising inefficiently. Thus, eve...